SPion Link™ is a breakthrough technology that extends the front-end of a mass spectrometer into a liquid handling station and seamlessly connects them to each other via a lossless ion transfer path. With SPion Link™, fully-automated mass spectrometry workflows can be developed to analyze samples directly from microtiter plates, such as 96-well plates, via custom disposable pipette tips. This provides significant advantages, particularly for clinical applications, where the amount of available sample is typically insignificant. SPion Link™ also minimizes reagent use and related costs, eliminates issues with cross-contamination and dead volumes, and allows for an unprecedented level of automation in mass spectrometry workflows essential in reproducibility of mass spectrometry data. It also reduces cost of analysis and minimizes exposure of lab personnel to biological samples that may be hazardous.